题目：细胞内看见未来：冷冻断层成像技术解析翻译全过程
主讲： 薛亮 博士
时间：2022年11月25日16:00-17:00
腾讯会议ID： 278-949-653
报告摘要：
To delineate molecular machines with atomic detail inside cells represents the future of structural biology. With cryo-electron tomography (cryo-ET) and advanced sub-tomogram analysis, I determined the ribosome average at 3.0 Å resolution inside Mycoplasma pneumoniae cells. Extensive sub-tomogram classification of ribosomes in native cells determined more than 13 intermediate structures (4-10 Å resolutions) along active translation, which not only visualizes the structural dynamics but also provides unique insights into their functional landscapes inside cells. Mapping back the classified ribosomes into the tomogram reveals the spatial and functional organization of translating ribosomes in the cell, which unraveled a local coordination mechanism within polysomes that is mediated by the ribosomal protein L9. Moreover, a variety of supramolecular assemblies formed by ribosomes and other molecular machines were captured, which include transcription-translation coupling complexes, ribosome- translocon complexes, and other supercomplexes that may only form within the cellular context. Furthermore, I resolved more than 17 ribosome intermediate structures in three different antibiotic-treated datasets, and demonstrated how the drug molecules drastically reshape structural and functional landscapes of the translation machinery inside bacterial cells. The works pushed limits of cryo-ET and sub-tomogram analysis, and herald a new era of integrative in-cell structural biology.
报告人简介：
Dr. Liang Xue received PhD from European Molecular Biology Laboratory(EMBL) in 2022. He achieved an in-cell ribosome map at 3 Å resolution using cryo-electron tomography (cryo-ET).
